H2N2V1, Main, Exploration, bibRecord, 001A70

Development of a mammalian cell (Vero) derived candidate influenza virus vaccine

Identifieur interne : 001A70 ( Main/Exploration ); précédent : 001A69; suivant : 001A71

Development of a mammalian cell (Vero) derived candidate influenza virus vaccine

Auteurs : O. Kistner [Autriche] ; P. N. Barrett [Autriche] ; W. Mundt [Autriche] ; M. Reiter [Autriche] ; S. Schober-Bendixen [Autriche] ; F. Dorner [Autriche]

Source :

Vaccine [ 0264-410X ] ; 1998.

RBID : ISTEX:1AE5DDAE72479A46D53ACAF0C702D8A3B7A6AB2F

English descriptors

Teeft :
- Adventitious viruses, Allantoic fluid, Assay, Biol, Blood cells, Cell bank, Cell culture, Cell culture medium, Cell line, Cell lines, Cell number, Chemical accurates, Chorioallantoic membrane, Continuous cell lines, Corresponding strains, Corresponding vero, Culture medium, Direct comparison, Effective dose, Elisa, Elsevier science, Embryonated, Embryonated chicken eggs, Embryonated eggs, Fermenter, Formalin, Gentle agitation, Hemagglutination, Hemagglutinin, Hlnl, Horseradish peroxidase, Host system, Human vaccines, Immunization, Industrial production, Infectious virus, Influenza, Influenza strains, Influenza subunit vaccine, Influenza vaccine, Influenza vaccine production, Influenza vaccines, Influenza virus, Influenza virus preparations, Influenza virus vaccine strains, Influenza viruses, Johannesburg, Kistner, Large quantities, Larger volume, Master cell bank, Maximum virus, Mdck cells, Microtitre plate, Monolayer cultures, More immunogenic, Mouse sera, Nanchang, Neuraminidase, Neuraminidase activity, Nibsc, Optical density, Passage level, Passaged, Peak fractions, Precipitation zones, Protamine sulphate precipitation, Purification procedure, Purification scheme, Purification steps, Rapid production, Reagent, Roller bottles, Room temperature, Specific sera, Standard dose, Sucrose gradient, Tissue culture, Titre, Transcriptase assay, Trypsin, Vaccine, Vaccine production, Vaccine strains, Vcro virus, Vero, Vero cell, Vero cell culture, Vero cell cultures, Vero cell line, Vero cell system, Vero cells, Vero nanchang, Vero vaccine, Viral, Viral contamination, Virology, Virus, Virus antigen, Virus preparations, Virus strains, Virus vaccine, Western blot analysis, Westernblot analysis, Wide range.

Abstract

Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.

Url:

https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/fulltext.pdf

DOI: 10.1016/S0264-410X(97)00301-0

Affiliations:

Autriche

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Le document en format XML

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<term>Assay</term>
<term>Biol</term>
<term>Blood cells</term>
<term>Cell bank</term>
<term>Cell culture</term>
<term>Cell culture medium</term>
<term>Cell line</term>
<term>Cell lines</term>
<term>Cell number</term>
<term>Chemical accurates</term>
<term>Chorioallantoic membrane</term>
<term>Continuous cell lines</term>
<term>Corresponding strains</term>
<term>Corresponding vero</term>
<term>Culture medium</term>
<term>Direct comparison</term>
<term>Effective dose</term>
<term>Elisa</term>
<term>Elsevier science</term>
<term>Embryonated</term>
<term>Embryonated chicken eggs</term>
<term>Embryonated eggs</term>
<term>Fermenter</term>
<term>Formalin</term>
<term>Gentle agitation</term>
<term>Hemagglutination</term>
<term>Hemagglutinin</term>
<term>Hlnl</term>
<term>Horseradish peroxidase</term>
<term>Host system</term>
<term>Human vaccines</term>
<term>Immunization</term>
<term>Industrial production</term>
<term>Infectious virus</term>
<term>Influenza</term>
<term>Influenza strains</term>
<term>Influenza subunit vaccine</term>
<term>Influenza vaccine</term>
<term>Influenza vaccine production</term>
<term>Influenza vaccines</term>
<term>Influenza virus</term>
<term>Influenza virus preparations</term>
<term>Influenza virus vaccine strains</term>
<term>Influenza viruses</term>
<term>Johannesburg</term>
<term>Kistner</term>
<term>Large quantities</term>
<term>Larger volume</term>
<term>Master cell bank</term>
<term>Maximum virus</term>
<term>Mdck cells</term>
<term>Microtitre plate</term>
<term>Monolayer cultures</term>
<term>More immunogenic</term>
<term>Mouse sera</term>
<term>Nanchang</term>
<term>Neuraminidase</term>
<term>Neuraminidase activity</term>
<term>Nibsc</term>
<term>Optical density</term>
<term>Passage level</term>
<term>Passaged</term>
<term>Peak fractions</term>
<term>Precipitation zones</term>
<term>Protamine sulphate precipitation</term>
<term>Purification procedure</term>
<term>Purification scheme</term>
<term>Purification steps</term>
<term>Rapid production</term>
<term>Reagent</term>
<term>Roller bottles</term>
<term>Room temperature</term>
<term>Specific sera</term>
<term>Standard dose</term>
<term>Sucrose gradient</term>
<term>Tissue culture</term>
<term>Titre</term>
<term>Transcriptase assay</term>
<term>Trypsin</term>
<term>Vaccine</term>
<term>Vaccine production</term>
<term>Vaccine strains</term>
<term>Vcro virus</term>
<term>Vero</term>
<term>Vero cell</term>
<term>Vero cell culture</term>
<term>Vero cell cultures</term>
<term>Vero cell line</term>
<term>Vero cell system</term>
<term>Vero cells</term>
<term>Vero nanchang</term>
<term>Vero vaccine</term>
<term>Viral</term>
<term>Viral contamination</term>
<term>Virology</term>
<term>Virus</term>
<term>Virus antigen</term>
<term>Virus preparations</term>
<term>Virus strains</term>
<term>Virus vaccine</term>
<term>Western blot analysis</term>
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<front><div type="abstract" xml:lang="en">Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.</div>
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Development of a mammalian cell (Vero) derived candidate influenza virus vaccine

Development of a mammalian cell (Vero) derived candidate influenza virus vaccine

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri